FORMATION OF AXILLARY SHOOTS OF CASTANEA SATIVA MILL, IN VITRO

A. KAMENICKA

Abstract


The work deals with the effects of two culture media on the formation of axillary shoots of Spanish chestnut (Castanea sativa Mill.) in vitro culture. Data presented indicate that the composition of culture medium is important for induction of axillary shoots. The mean number of axillary shoots on the S2 medium was 2.95 per explant. By containing calcium to S, medium the number of produced shoots increased significantly (4.28 per explant) and formation of vitrified shoots decreased and drying of tip was suppressed. In the WPM, medium containing calcium, and callus formation and the elongation growth of shoots was suppressed. The mean number of axillary shoots on the WPM1 medium was 4.43 per explant in comparison with WPM2 medium which was 2.66 per explant.


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DOI: https://doi.org/10.13133/2239-3129/15439

Annali di Botanica is published by the Department of Environmental Biology - University La Sapienza of Rome, Italy. The journal is printed by Sapienza Università Editrice – Sapienza Università di Roma.

ISSN 0365-0812 (print)

ISSN 2239-3129 (online)